Hi,
Yep definitely hormones!! I have had the same trouble, one of my queens is pregnant & I have an upcoming queen who is almost 10 months. Over the last couple of weeks my queen who is pregnant seems to be picking on my upcoming queen. I have now seperated my queen that is due & have put her in my bedroom which I do anyway before the birth. She has about 3 weeks to go so have only put her in my room a little earlier.
There was more hissing & growling so thought it best to seperate before anything awful happened.
Coule you not put the queen who is further along in your room perhaps? Please let us know how you get on
