Hi and welcome to the forum!
Well from my own experience, I know it is possible for a female to get pregnant at this age, but I cannot comment on the likelyhood of it happening to a Tonkinese.

From my understanding of cats, I believe the girl 'calls' the boy to mate, this is normally very noticable, the girls meow very loudly, roll around on the floor and stick their bums in the air.

I would suggest you do seperate them for the night, and any time they are alone. Plenty of people re-home one kitten at a time, and they manage being on their own, so I'm sure yours will be fine. The last thing you want if for your girl to be pregnant at this age, least of all with her brothers babies.

I suggest you speak to your vet tomorrow about bringing them in, they may be able to spay/neuter one of them early. But I am pretty sure there are no vets in my area that would do them this young.

I have heard some of the breeders on here talking about stud pants, these are to stop the un-neutered boys spraying inside the house, I wonder if they might help keep your chaps little chap away from your girl.