Puppy’s crying at night: sorry its so long
It is entirely natural for a new puppy to call out for someone to come when they are left on their own. Your puppy is just following its instinct, by calling out to you. If the puppy is very distressed and clearly emotionally upset, then suggest allowing her to sleep in your room, but within an indoor crate. Over a period of time you can move the crate in stages: I.E - the kitchen. The first thing you need to do is ignore her making noise and the last thing you want to do is go down and scold her because the fact that you appeared in response to the cries, will reinforced her crying instinct. If noise never elicits a response, then the pup will learn that this method of communication does not work. What never works is waiting whilst the puppy makes more and more noise then finally becoming annoyed or exasperated and going to the puppy. All that would teach the pup was being especially PERSISTENT really works! Punishment does NOT teach your dog to be quiet, any more than it would a baby crying. Punishment or anger would stress him even more and could create behaviour problems or even affect your future relationship. You can help your puppy accept the separation more quickly by introducing the area you want the pup to stay gently and carefully. Feed favourite treats in this area, if you are using a crate then you can leave a stuffed Bone or Kong in the crate as you go up to bed. Leave a radio on low. Buy a * DAP Diffuser to help the puppy settle in, and a hot pad which when heated for 5 minutes in a microwave stays warm and comfortable for up to 12 hours. Correctly introduced it speeds up the toilet training process, gives the pup a secure and comfortable den and a bolthole in times of stress. It can also be an invaluable asset when the pup gets older. Situations where a dog may need the ability to accept and relax in a crate include:
Recovery from medical problems, travel, emergency, and adjustment to a new home or a new family.

Obviously the best time to introduce a crate is as puppies. * D.A.P Diffusers: In mammals all lactating females release substances which are called appeasing pheromones, the function is to reassure and calm their offspring, In the bitch these are produced 3-5 days post whelping by the sebaceous glands of the belly near the milk glands. They are believed to enhance attachment between mother and pups and to reassure and comfort. As the pup explores its new environment it will come across new stimuli which set off emotional reactions and stresses. The pup reacts by looking for mum with her reassuring odour which stabilises its emotional state. Research has shown that these reassuring properties last well into adulthood. They appear to modulate both emotional state and social interaction throughout the dog’s life. The DAP Diffuser standing for Dog Appeasing Pheromone Diffuser is a chemical mimic of the mothers natural pheromone and is supplied in spray and plug in varieties. I prefer the plug in as it has a culminative effect constantly seeding the area with calming smells. I have used this with a previous puppy i owned.

you can also try a snuggle puppy, they cost about £25 and come in different breed varietys! You can now also get them for kittens too!
heres the website link:Home

hope this helps

puppies crying at night is not copy writted or copied and pasted. it has been changed into my own words so that there is not problems with what i have posted. clearly there has been some problems with a few other posts but all have been sorted out and changed completely.