Hi Mo,

Thanks for your response. In India, we loose many pups because of this disease.
Subsequent to your reply I had contacted one of my pathologist friend, who advised that as a thumb rule each 5 ml of blood can produce around 3 ml of Serum. Ideally then, 15 to 20 ml of blood would have served the purpose for the eight puppies.
My question is, is there any other way of spunning the blood involved for this particular treatment, which may require higher quantity of blood for producing the serum! Requesting your help and advise from any of your friends knowledgeable on the issue, so that we can save some lives here also. Recently I lost one of my Golden Retriever pups because of this disease, which compelled me to dig out information on addressing the issue.

rgds avijit